African leaf (Vernonia amygdalina Del.) is one of the medicinal plants that is widely used by the community as traditional medicine. The leaf part of the African leaf plant (Vernonia amygdalina Del.) which contains alkaloids, phenols and steroids as an antibacterial for Staphylococcus aureus which causes skin infections. The purpose of this study was to determine the antibacterial activity of African leaves (Vernonia amygdalina Del.) at concentrations of 20%, 40%, 60% on the growth of Staphylococcus aureus bacteria. This research is a laboratory experiment conducted at the Jayapura Ministry of Health Polytechnic Phytochemical Laboratory and Uncen Biology Laboratory in May–June 2023. African leaves were extracted using the infusion method with aquadest solvent. The content of African leaf compounds can be seen using the phytochemical screening method. The results of the phytochemical screening showed that the positive African leaf infusion contained alkaloids, phenols, and steroids/triterpenoids which function as antibacterials. To test its activity, the disc method was used with the antibiotic Ciprofloxacin as a positive control and aquadest as a negative control. The results of the research on antibacterial activity that at a concentration of 20% the diameter was 6.54 mm, at a concentration of 40% the diameter was 7.94 mm, and at a concentration of 60% the diameter was 9.24 mm. The existence of an inhibition zone that occurs in the Staphylococcus aureus bacteria proves that African leaves contain active compounds that are antibacterial. In this study it can be concluded that there is antibacterial activity of African leaf infusion (Vernonia amygdalina Del.) on the growth of Staphylococcus aureus at concentrations of 20%, 40%, and 60% with the category of moderate inhibition zone.